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This project will use cultured peripheral blood mononuclear cells (PBMCs) from HIV-1-infected patients for assessing the induction of DNA damage and the modulation of cytokine and chemokine expression following exposure to HIV-1 envelope glycoprotein gp120. The studies will test a model system that employs a primary culture of monocyte-derived macrophages, which have been previously shown to be a useful model for studying immune functions of monocyte-derived macrophages in HIV infection. The proposed studies will address the hypothesis that HIV-1-infected macrophages can induce DNA damage in lymphocytes and that this induction of damage can modulate the expression of cytokines and chemokines. The specific aims are: 1) To assess the induction of DNA damage and the modulation of cytokines and chemokines in cultured peripheral blood mononuclear cells (PBMCs) following exposure to HIV-1 envelope glycoprotein (gp120) by cells from HIV-1-infected individuals. 2) To determine the induction of DNA damage and the modulation of cytokines and chemokines in macrophages, following exposure to HIV-1 envelope glycoprotein. 3) To determine whether the infection of macrophages with HIV-1 alters the expression of cytokines and chemokines and DNA damage in macrophages.Thailand 'WannaCry' attack: Hospitals hit Published duration 23 May 2017 image copyright Getty Images image caption Hospitals and clinics in Thailand are taking part in the attacks A computer virus attack hitting more than 300 healthcare organisations in Thailand is causing disruption in many parts of the country. Cybersecurity firm Symantec says the virus, known as WannaCry or Wana Decryptor, will eventually "explode". 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